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Unlike viral IRESs, cellular IRESs may exhibit weak secondary structures , suggesting that RNA structure is not the sole determinant of the IRES activity.
Northern blotting and signal quantification RNA was separated on 1.
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Therefore, we propose that BBV-seq sequence constitutes a previously undescribed CITE element that in yeast recruits eIF4G1 for efficient binding of 43S PIC and positioning the 40S subunit for scanning to identify the start codon of the downstream gene Fig.
Similarly to IRESs, CITEs can initiate translation without a full set of translation initiation factors.
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